Título

The search for cryptic L-Rhamnosyltransferases on the Sporothrix schenckii genome

Autor

Hector M. Mora-Montes

Karina García-Gutiérrez

Laura Cristina García Carnero

Nancy Lozoya-Perez

Jorge Humberto Ramírez Prado

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

datasetDOI/https://doi.org/10.3390/jof8050529

Materias

FUNGAL CELL-WALL - (AUTORES) GLYCANS - (AUTORES) RHAMNOCONJUGATES - (AUTORES) RHAMNOSYLTRANSFERASE - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA VEGETAL (BOTÁNICA) - (CTI) ECOLOGÍA VEGETAL - (CTI) ECOLOGÍA VEGETAL - (CTI)

Resumen o descripción

The fungal cell wall is an attractive structure to look for new antifungal drug targets and for understanding the host-fungus interaction. Sporothrix schenckii is one of the main causative agents of both human and animal sporotrichosis and currently is the species most studied of the Sporothrix genus. The cell wall of this organism has been previously analyzed, and rhamnoconjugates are signature molecules found on the surface of both mycelia and yeast-like cells. Similar to other reactions where sugars are covalently linked to other sugars, lipids, or proteins, the rhamnosylation process in this organism is expected to involve glycosyltransferases with the ability to transfer rhamnose from a sugar donor to the acceptor molecule, i.e., rhamnosyltransferases. However, no obvious rhamnosyltransferase has thus far been identified within the S. schenckii proteome or genome. Here, using a Hidden Markov Model profile strategy, we found within the S. schenckii genome five putative genes encoding for rhamnosyltransferases. Expression analyses indicated that only two of them, named RHT1 and RHT2, were significantly expressed in yeast-like cells and during interaction with the host. These two genes were heterologously expressed in Escherichia coli, and the purified recombinant proteins showed rhamnosyltransferase activity, dependent on the presence of UDP-rhamnose as a sugar donor. To the best of our knowledge, this is the first report about rhamnosyltransferases in S. schenckii. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Fecha de publicación

2022

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2854

Formato

application/pdf

Fuente

Journal of Fungi, 8(5), 529, 2022.

Idioma

Inglés

Sugerencia de citación

Mora-Montes, H.M.; García-Gutiérrez, K.; García-Carnero, L.C.; Lozoya-Pérez, N.E.; Ramirez-Prado, J.H. The search for cryptic L-Rhamnosyltransferases on the Sporothrix schenckii genome. J. Fungi 2022, 8, 529. https://doi.org/10.3390/jof8050529

Repositorio Orígen

Repositorio Institucional CICY

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