Screening of xylose utilizing and high lipid producing yeast strains as a potential candidate for industrial application

Linnea Qvirist RICARDO VAZQUEZ JUAREZ Thomas Andlid (2022, [Artículo])

"Background: Sustainable production of oil for food, feed, fuels and other lipid-based chemicals is essential to meet the demand of the increasing human population. Consequently, novel and sustainable resources such as lignocel- lulosic hydrolysates and processes involving these must be explored. In this paper we screened for naturally-occurring xylose utilizing oleaginous yeasts as cell factories for lipid production, since pentose sugar catabolism plays a major role in efcient utilization of lignocellulosic feedstocks. Glycerol utilization, which is also benefcial in yeast-based oil production as glycerol is a common by-product of biodiesel production, was investigated as well. Natural yeast isolates were studied for lipid accumulation on a variety of substrates, and the highest lipid accumulating strains were further investigated in shake fask cultivations and fermenter studies on xylose and hydrolysate. Results: By collecting leaves from exotic plants in greenhouses and selective cultivation on xylose, a high frequency of oleaginous yeasts was obtained (>40%). Diferent cultivation conditions lead to diferences in fatty acid contents and compositions, resulting in a set of strains that can be used to select candidate production strains for diferent purposes. In this study, the most prominent strains were identifed as Pseudozyma hubeiensis BOT-O and Rhodosporidium toruloides BOT-A2. The fatty acid levels per cell dry weight after cultivation in a nitrogen limited medium with either glucose, xylose or glycerol as carbon source, respectively, were 46.8, 43.2 and 38.9% for P. hubeiensis BOT-O, and 40.4, 27.3 and 42.1% for BOT-A2. Furthermore, BOT-A2 accumulated 45.1% fatty acids per cell dry weight in a natural plant hydrolysate, and P. hubeiensis BOT-O showed simultaneous glucose and xylose consumption with similar growth rates on both carbon sources. The fatty acid analysis demonstrated both long chain and poly-unsaturated fatty acids, depending on strain and medium. Conclusions: We found various natural yeast isolates with high lipid production capabilities and the ability to grow not only on glucose, but also xylose, glycerol and natural plant hydrolysate. R. toruloides BOT-A2 and P. hubeiensis BOT-O specifcally showed great potential as production strains with high levels of storage lipids and comparable growth to that on glucose on various other substrates, especially compared to currently used lipid production strains..."

Microbial lipids, Oleaginous yeast, Lignocellulose, Pseudozyma hubeiensis, Rhodosporidium toruloides BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA MICROBIOLOGÍA

Publisher Correction: Gut microbiota mediate caffeine detoxification in the primary insect pest of coffee (Nature Communications, (2015), 6, 1, (7618), 10.1038/ncomms8618)

Javier A. Ceja-Navarro Fernando E. Vega Ulas Karaoz Zhao Hao Petr Kosina Francisco Infante Trent Northen Eoin Brodie (2023, [Artículo])

Antibiotic Frass Non Antibiotic CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MICROBIAL FLORA PEST INSECTS HYPOTHENEMUS HAMPEI CAFFEINE

In vitro Digestibility of Yarrowia lipolytica Yeast and Growth Performance in Whiteleg Shrimp Litopenaeus vannamei

ANA RUTH ALVAREZ SANCHEZ CLAUDIO HUMBERTO MEJIA RUIZ Héctor Gerardo Nolasco Soria Alberto Peña Rodríguez (2018, [Artículo])

"Marine yeasts used in aquaculture disease control can also be an important protein source for improving feeding and nutrition of crustaceans. Yarrowia lipolyticca has been studied for its capacity to secrete heterologous proteins and high content of unsaturated fatty acids, beta-glucan, and mannane polymers in the cell wall. We measured in vitro digestibility of Y. lipolyticca by whiteleg shrimp Litopenaeus vannamei digestive enzymes, and an in vivo assay of Y. lipolytica in feed onwhiteleg shrimp growth. We found that digestive gland enzymes of shrimp digest Y. lipolytica, based on reduced optical density of a yeast suspension. Digestion was –0.00236 ± 0.00010 OD U min–1 for intact cells and –0.00325 ± 0.00010 OD U min–1 for lysed cells. Release of reducing sugars in intact cells (5.3940 ± 0.1713 μmol h–1), and lysed cells (0.8396 ± 0.2251 μmol h–1) was measured. Digestive gland treatment significantly reduced cell viability (near 100%), relative to the control. Electron microscopy shows that the cell wall of Y. lipolytica exposed to the digestive gland enzymes was severely damaged. Shrimp diet containing Y. lipolytica resulted in significantly higher weight gain and specific growth rate of whiteleg shrimp."

Marine yeast, cell digestibility, cell viability, turbidimetry, reduced sugars BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOQUÍMICA BIOQUÍMICA DE ALIMENTOS BIOQUÍMICA DE ALIMENTOS