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Explorando microbiomas extremófilos para descubrir enzimas inéditas que transforman almidón

Víctor Manuel Carballo Uicab XITLALLI MONTSERRAT ROMERO JIMENEZ JORGE IVAN ARANDA CARABALLO SARA GUILLERMINA CENTENO LEIJA (2022, [Artículo])

Las cicloglucanotransferasas (CGTasas) transforman el polímero almidón en nanomoléculas anfipáticas de interés biotecnológico llamadas ciclodextrinas. Aunque las CGTasas termófilas son ideales para procesos industriales, el 75 % de las CGTasas caracterizadas pertenecen a bacterias mesófilas de la sobreexplorada clase Bacilli. Recientemente la minería de datos (meta)genómicos ha permitido detectar nuevas CGTasas presentes en nichos ecológicos inexplorados. El estudio de éstas CGTasas inéditas, ofrece pistas sobre su papel fisiológico en microbiomas extremófilos y su caracterización abre la oportunidad de ampliar el conocimiento sobre su estructura, mecanismos funcionales y proponer modificaciones racionales que incrementen su eficiencia para obtener productos de valor agregado.

CICLODEXTRINAS CICLOGLUCANOTRANSFERASAS METABOLISMO DE CARBOHIDRATOS MINERIA DE (META)GENOMAS TERMOENZIMAS BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) ECOLOGÍA VEGETAL ECOLOGÍA VEGETAL

¿Desvinculación o decrecimiento? Un marco analítico para abordar la transición socioecológica desde los estudios urbanos

Jerónimo Aurelio Díaz Marielle (2023, [Artículo])

Artículo número 1 de la Sección Articulos de Investigación.

La transición socioecológica se perfila como un nuevo paradigma científico centrado en las interacciones sociedad-naturaleza y es, al mismo tiempo, la punta de lanza de una nueva agenda urbana y ambiental que no está exenta de contradicciones y dilemas. Con un pie en la divulgación de los conceptos y las metodologías que conforman este paradigma, y con otro en el análisis sociológico de los nuevos ecologismos, el artículo ofrece una revisión de dos corrientes de ecología política que buscan orientar el sentido de la transición: la desvinculación ecológica y el decrecimiento. La primera coloca sus expectativas en el desarrollo tecnológico y el mercado, es favorable al modelo de la ciudad compacta y promueve las llamadas soluciones basadas en la naturaleza. La segunda reconoce el valor de los saberes urbanos vernáculos, pero apela a la descentralización de las urbes y pugna por una reducción equitativa y democrática de los estándares de vida de la población. El artículo concluye que los estudios urbanos (en particular la sociología urbana y la planeación territorial) pueden contribuir a comprender el funcionamiento de los metabolismos urbanos y aportar soluciones a las dificultades que impiden el tránsito hacia una sociedad sustentable, esto es, una sociedad que sea capaz de autolimitarse, ajustando su metabolismo a los ciclos y a los tiempos que requiere la naturaleza para regenerarse.

The socio-ecological transition is emerging as a new scientific paradigm focused on the interactions between society and nature, and at the same time, it is the spearhead of a new urban and environmental agenda. With one foot in the dissemination of the concepts and methodologies that make up this paradigm, and with the other in the sociological analysis of the new environmental movements, the article offers a review of two political ecology forces that seek to guide the direction of the transition: ecological decoupling and degrowth. The former places its expectations on technological development and the market, is favorable to the compact city model and promotes so-called nature-based solutions. The latter recognizes the value of vernacular urban knowledge, but calls for the decentralization of cities and advocates for an equitable and democratic reduction of the population standards of living. The article concludes that urban studies (particularly urban sociology and territorial planning) can contribute to understanding the functioning of urban metabolisms and providing ways to address social inertia regarding climate change.

Cambio climático, metabolismo urbano, planeación territorial. Climate change, urban metabolism, territorial planning. HUMANIDADES Y CIENCIAS DE LA CONDUCTA CIENCIAS DE LAS ARTES Y LAS LETRAS ARQUITECTURA URBANISMO

High quality diet improves lipid metabolic profile and breeding performance in the blue-footed booby, a long-lived seabird

ERICK GONZALEZ MEDINA (2018, [Artículo])

Understanding the role of diet in the physiological condition of adults during reproduction and hence its effect on reproductive performance is fundamental to understand reproductive strategies in long-lived animals. In birds, little is known about the influence of the quality of food consumed at the beginning of the reproductive period and its short-term effects on reproductive performance. To assess the role of diet in the physiological condition of female blue-footed booby, Sula nebouxii (BFBO), during reproduction we evaluated whether individual differences in diet (assessed by using δ13C and δ15N values of whole blood from female birds and muscle tissue of the principal prey species) prior to egg laying and during incubation influenced their lipid metabolic profile (measured as triglyceride levels and C:N ratio) and their reproductive performance (defined by laying date, clutch size and hatching success). Females with higher δ15N values in their blood during the courtship and incubation periods had a higher lipid metabolic profile, earlier laying date, greater clutch size (2–3 eggs) and higher hatching success. Females that laid earlier and more eggs (2–3 eggs) consumed more Pacific anchoveta (Cetengraulis mysticetus) and Pacific thread herring (Opisthonema libertate) than did other females. These two prey species also had high amounts of lipids (C:N ratio) and caloric content (Kcal/g fresh weight). The quality of food consumed by females at the beginning of reproduction affected their physiological condition, as well as their short-term reproductive performance. Our work emphasizes the importance of determining the influence of food quality during reproduction to understand the reproductive decisions and consequences in long-lived animals. © 2018 González-Medina et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

nitrogen 15, triacylglycerol, lipid, animal experiment, Article, breeding, carbon nitrogen ratio, clutch size, controlled study, courtship, diet, egg laying, female, food intake, hatching, lipid metabolism, muscle tissue, nonhuman, prey, reproduction CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Protein retention assessment of four levels of poultry by-product substitution of fishmeal in rainbow trout (Oncorhynchus mykiss) diets using stable isotopes of nitrogen (δ15N) as natural tracers

DANIEL BADILLO ZAPATA (2014, [Artículo])

This is second part from an experiment where the nitrogen retention of poultry by-product meal (PBM) compared to fishmeal (FM) was evaluated using traditional indices. Here a quantitative method using stable isotope ratios of nitrogen (δ15N values) as natural tracers of nitrogen incorporation into fish biomass is assessed. Juvenile rainbow trout (Oncorhynchus mykiss) were fed for 80 days on isotopically distinct diets in which 0, 33, 66 and 100% of FM as main protein source was replaced by PBM. The diets were isonitrogenous, isolipidic and similar in gross energy content. Fish in all treatments reached isotopic equilibrium by the end of the experiment. Two-source isotope mixing models that incorporated the isotopic composition of FM and PBM as well as that of formulated feeds, empirically derived trophic discrimination factors and the isotopic composition of fish that had reached isotopic equilibrium to the diets were used to obtain a quantitative estimate of the retention of each source of nitrogen. Fish fed the diets with 33 and 66% replacement of FM by PBM retained poultry by-product meal roughly in proportion to its level of inclusion in the diets, whereas no differences were detected in the protein efficiency ratio. Coupled with the similar biomass gain of fishes fed the different diets, our results support the inclusion of PBM as replacement for fishmeal in aquaculture feeds. A re-feeding experiment in which all fish were fed a diet of 100% FM for 28 days indicated isotopic turnover occurred very fast, providing further support for the potential of isotopic ratios as tracers of the retention of specific protein sources into fish tissues. Stable isotope analysis is a useful tool for studies that seek to obtain quantitative estimates of the retention of different protein sources. © 2014 Badillo et al.

nitrogen 15, nitrogen, protein intake, animal behavior, animal experiment, animal food, animal tissue, aquaculture, Article, biomass, controlled study, energy metabolism, food composition, juvenile animal, nonhuman, poultry by product meal, protein a CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

The Pacific harbor seal gut microbiota in Mexico: Its relationship with diet and functional inferences

ARLETTE MARIMAR PACHECO SANDOVAL (2019, [Artículo])

Diet is a primary driver of the composition of gut microbiota and is considered one of the main routes of microbial colonization. Prey identification is fundamental for correlating the diet with the presence of particular microbial groups. The present study examined how diet influenced the composition and function of the gut microbiota of the Pacific harbor seal (Phoca vitulina richardii) in order to better understand the role of prey consumption in shaping its microbiota. This species is a good indicator of the quality of the local environment due to both its foraging and haul-out site fidelity. DNA was extracted from 20 fecal samples collected from five harbor seal colonies located in Baja California, Mexico. The V4 region of 16S rRNA gene was amplified and sequenced using the Illumina technology. Results showed that the gut microbiota of the harbor seals was dominated by the phyla Firmicutes (37%), Bacteroidetes (26%) and Fusobacteria (26%) and revealed significant differences in its composition among the colonies. Funtional analysis using the PICRUSt software suggests a high number of pathways involved in the basal metabolism, such as those for carbohydrates (22%) and amino acids (20%), and those related to the degradation of persistent environmental pollutants. In addition, a DNA metabarcoding analysis of the same samples, via the amplification and sequencing of the mtRNA 16S and rRNA 18S genes, was used to identify the prey consumed by harbor seals revealing the consumption of prey with mainly demersal habits. Functional redundancy in the seal gut microbiota was observed, irrespective of diet or location. Our results indicate that the frequency of occurrence of specific prey in the harbor seal diet plays an important role in shaping the composition of the gut microbiota of harbor seals by influencing the relative abundance of specific groups of gut microorganisms. A significant relationship was found among diet, gut microbiota composition and OTUs assigned to a particular metabolic pathway. © 2019 Pacheco-Sandoval et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

RNA 16S, RNA 18S, amino acid analysis, animal food, Article, bacterium colony, Bacteroidetes, basal metabolic rate, biodegradation, controlled study, DNA barcoding, feces analysis, Firmicutes, Fusobacteria, intestine flora, metabolism, Mexico, microb BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) BIOLOGÍA ANIMAL (ZOOLOGÍA)